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Q. No.Enzymes that catalyze the removal of nucleotides in either the 5-prime to 3-prime or the 3-prime to 5-prime direction from the ends of single-stranded and/or double-stranded DNA are called:
| 1. | endonuclease | 2. | exonuclease |
| 3. | ligase | 4. | polymerase |
Subtopic: Introduction & History: I | Introduction & History: II |
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
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Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism constitute:
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
1. Biotechnology
2. Genetic engineering
3. Bioprocess engineering
4. Genomics
Subtopic: Introduction & History |
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'Heat shock' method in bacterial transformation:
| 1. | allows selection of transformants |
| 2. | promotes uptake of DNA through membrane transport proteins |
| 3. | creates pores in the bacterial plasma membrane and allows plasmid DNA to enter the bacterial DNA. |
| 4. | coagulates DNA in the bacterial cell. |
Subtopic: Transforming Plant & Animal Cell |
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Which of the following is not required in the preparation of a recombinant DNA molecule?
| 1. | Restriction endonuclease | 2. | DNA ligase |
| 3. | DNA fragments | 4. | E.coli |
Subtopic: Tools | General Design of an rDNA experiment |
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Why is ethidium bromide used to stain DNA in gel electrophoresis?
| 1. | It increases the DNA’s mobility through the gel. |
| 2. | It condenses the DNA to allow it to enter the gel. |
| 3. | It fluoresces under UV light, allowing visualization of DNA bands. |
| 4. | It cleaves DNA at specific bases, allowing it to be visualized. |
Subtopic: Separation and Isolation of DNA fragments |
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Human Genome Project |
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The advantage of using DNA polymerases from thermophilic organisms in PCR is that:
| 1. | the DNA polymerases of these bacteria are much faster than those from other organisms |
| 2. | the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands |
| 3. | the DNA polymerases of these bacteria never make mistakes while replicating DNA |
| 4. | all of the above |
Subtopic: Process of Biotech |
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In the name ‘Sma I’, which of the following is missing?
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
1. Indicative of generic name
2. Indicative of specific name
3. Indicative of strain
4. Order of identification in the bacterium
Subtopic: General Design of an rDNA experiment |
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The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
Subtopic: Polymerase Chain Reaction: PCR |
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