1. | would not be able to multiply itself in the progeny cells of the organism. |
2. | would be transcribed but the transcript will not be translated. |
3. | would express as different proteins from the organism it was derived from. |
4. | would be invariably destroyed by the cytosolic endonucleases in eukaryotic cells. |
1. | |
2. | |
3. | |
4. |
I: | Plasmid |
II: | Phage DNA |
Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. |
Reason (R): | All bacterial cells already have plasmids and will not normally pick up another. |
1. | Both (A) and (R) are True and (R) correctly explains the (A). |
2. | (A) is True; (R) is False |
3. | (A) is False; (R) is False |
4. | Both (A) and (R) are True but (R) does not correctly explain the (A) |
Statement I: | There is a need for the recombinant DNA to be expressed in heterologous host. |
Statement II: | In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. |
Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | (A) is True; (R) is False |
3. | (A) is False; (R) is False |
4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
1. | Gene gun | 2. | Gene probe |
3. | Snip | 4. | Selectable marker |