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Q. No.Consider the given two statements:
Assertion (A):
In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Reason (R):
Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1.
Both (A) and (R) are True and (R) correctly explains (A).
2.
(A) is True; (R) is False
3.
(A) is False; (R) is False
4.
Both (A) and (R) are True but (R) does not correctly explain (A).
Subtopic: Cloning Vector |
91%
From NCERT
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A single-stranded nucleic acid fragment, tagged with a radioactive molecule, that interacts with a complementary sequence of a target nucleic acid is called a:
| 1. | Gene gun | 2. | Gene probe |
| 3. | Snip | 4. | Selectable marker |
Subtopic: Molecular Diagnosis |
87%
From NCERT
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
| 1. | 1000 copies | 2. | One lakh copies |
| 3. | One million copies | 4. | One billion copies |
Subtopic: Polymerase Chain Reaction: PCR |
86%
From NCERT
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic: Cloning Vector |
84%
From NCERT
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In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
| 1. | Pvu I | 2. | BamH I |
| 3. | Pst I | 4. | Pvu II |
Subtopic: Selectable Markers: III |
80%
From NCERT
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Consider the given two statements:
| Assertion (A): | The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. |
| Reason (R): | The normal E. coli cells do not carry resistance against any of these antibiotics. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Selectable Markers: I |
54%
From NCERT
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Biotechnology has been defined as ‘the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services, by:
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
Subtopic: Introduction & History: I |
90%
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
73%
From NCERT
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Maintenance of sterile ambience in chemical engineering processes to enable growth of only the desired microbe/eukaryotic cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes, etc is called as:
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
Subtopic: Introduction & History: II |
85%
From NCERT
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The ‘sticky ends’ produced in the fragments of DNA produced by some restriction enzymes are composed of:
| 1. | methyl groups | 2. | telomeres |
| 3. | unpaired bases | 4. | Guanine rich repeats |
Subtopic: Restriction Enzymes - Main Enzymes |
71%
From NCERT
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