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Q. No.In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
1.
Pvu I
2.
BamH I
3.
Pst I
4.
Pvu II
Subtopic: Selectable Markers: III |
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Consider the given two statements:
| Assertion (A): | The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. |
| Reason (R): | The normal E. coli cells do not carry resistance against any of these antibiotics. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Selectable Markers: I |
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Biotechnology has been defined as ‘the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services, by:
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
Subtopic: Introduction & History: I |
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
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Maintenance of sterile ambience in chemical engineering processes to enable growth of only the desired microbe/eukaryotic cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes, etc is called as:
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
Subtopic: Introduction & History: II |
85%
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The ‘sticky ends’ produced in the fragments of DNA produced by some restriction enzymes are composed of:
| 1. | methyl groups | 2. | telomeres |
| 3. | unpaired bases | 4. | Guanine rich repeats |
Subtopic: Restriction Enzymes - Main Enzymes |
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Consider the given two statements:
| Assertion (A): | DNA cannot pass through cell membranes. |
| Reason (R): | DNA is a hydrophobic molecule. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Separation and Isolation of DNA fragments |
68%
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic: Cloning Vector |
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
| 1. | 1000 copies | 2. | One lakh copies |
| 3. | One million copies | 4. | One billion copies |
Subtopic: Polymerase Chain Reaction: PCR |
86%
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Consider the given two statements:
| Assertion (A): | The discovery of restriction enzymes led to the birth of recombinant DNA technology as a discipline. |
| Reason (R): | Restriction enzymes help in the precise cleavage of DNA at specific recognition sites, facilitating the insertion of foreign genes into plasmids. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation for (A) |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation for (A). |
| 3. | (A) is True, but (R) is False. |
| 4. | (A) is False, but (R) is True. |
Subtopic: Restriction Enzymes - Main Enzymes |
88%
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