Assertion (A): | The discovery of restriction enzymes led to the birth of recombinant DNA technology as a discipline. |
Reason (R): | Restriction enzymes help in the precise cleavage of DNA at specific recognition sites, facilitating the insertion of foreign genes into plasmids. |
1. | Both (A) and (R) are True and (R) is the correct explanation for (A) |
2. | Both (A) and (R) are True but (R) is not the correct explanation for (A). |
3. | (A) is True, but (R) is False. |
4. | (A) is False, but (R) is True. |
1. | after the scientists who discover them. |
2. | based on the bacteria from which they are derived. |
3. | after the bacteriophage whose DNA is cut by them. |
4. | There is no convention or principles for naming restriction enzymes. |
1. | pUC101 | 2. | EcoR I |
3. | BamH I | 4. | Sma I |
1. | Chelation | 2. | Centrifugation |
3. | Elution | 4. | Hybridisation |
1. | endonuclease | 2. | exonuclease |
3. | ligase | 4. | polymerase |
1. | Transduction | 2. | Conjugation |
3. | Transformation | 4. | Translation |
1. | Cleaving of phosphodiester bond in DNA by the enzyme |
2. | Cutting of DNA at specific position only |
3. | Prevention of the multiplication of bacteriophage by the host bacteria |
4. | All of the above |
1. | allows selection of transformants |
2. | promotes uptake of DNA through membrane transport proteins |
3. | creates pores in the bacterial plasma membrane and allows plasmid DNA to enter the bacterial DNA. |
4. | coagulates DNA in the bacterial cell. |
Assertion (A): | DNA cannot pass through cell membranes. |
Reason (R): | DNA is a hydrophobic molecule. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
3. | (A) is True; (R) is False |
4. | Both (A) and (R) are False |
Consider the given two statements
Assertion (A): | In agar gel electrophoresis, smaller DNA fragments move farther than the larger DNA fragments. |
Reason (R): | In agar gel electrophoresis, DNA fragments separate according to their size due to the sieving effect of the agarose gel. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
3. | (A) is True, (R) is False. |
4. | (A) is False, (R) is False. |