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Q. No.Enzymes that catalyze the removal of nucleotides in either the 5-prime to 3-prime or the 3-prime to 5-prime direction from the ends of single-stranded and/or double-stranded DNA are called:
1.
endonuclease
2.
exonuclease
3.
ligase
4.
polymerase
Subtopic: Introduction & History: I | Introduction & History: II |
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The process by which a virus transfers genetic material from one bacterium to another is called:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
Subtopic: Tools |
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Restriction Enzymes - Main Enzymes |
From NCERT
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'Heat shock' method in bacterial transformation:
| 1. | allows selection of transformants |
| 2. | promotes uptake of DNA through membrane transport proteins |
| 3. | creates pores in the bacterial plasma membrane and allows plasmid DNA to enter the bacterial DNA. |
| 4. | coagulates DNA in the bacterial cell. |
Subtopic: Transforming Plant & Animal Cell |
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Consider the given two statements
| Assertion (A): | In agar gel electrophoresis, smaller DNA fragments move farther than the larger DNA fragments. |
| Reason (R): | In agar gel electrophoresis, DNA fragments separate according to their size due to the sieving effect of the agarose gel. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is False. |
Subtopic: Separation and Isolation of DNA fragments |
88%
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Plasmids
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | are inherited from one generation to the next. |
| II: | may carry genes that give their host a selective advantage. |
| III: | can render bacteria drug-resistant. |
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Cloning Vector |
80%
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Consider the two statements:
| Assertion (A): | Restriction enzymes produced by bacterial cells do not harm their own DNA |
| Reason (R): | Bacterial DNA are single-stranded molecules. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is True |
Subtopic: Restriction Enzymes - Main Enzymes |
79%
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Consider the given two statements:
| I: | Transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). |
| II: | Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools |
68%
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The origin of replication sequence in a plasmid:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | is a sequence from where replication starts |
| II: | is a sequence responsible for controlling the copy number of linked DNA |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Cloning Vector |
95%
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Restriction Enzymes - Main Enzymes |
57%
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