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Q. No.The origin of replication sequence in a plasmid:
I:
is a sequence from where replication starts
II:
is a sequence responsible for controlling the copy number of linked DNA
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Cloning Vector |
96%
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Restriction Enzymes - Main Enzymes |
57%
From NCERT
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What is a key difference between traditional hybridization techniques and recombinant DNA technology?
| 1. | Traditional hybridization involves sexual reproduction between plants, while recombinant DNA technology involves the chemical synthesis of DNA. |
| 2. | Traditional hybridization can only combine DNA from similar species, while recombinant DNA technology can combine DNA from any species. |
| 3. | Recombinant DNA technology uses live animals, whereas traditional hybridization uses only plant species. |
| 4. | Traditional hybridization is faster than recombinant DNA technology. |
Subtopic: Principles of Biotechnology |
62%
From NCERT
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Why is ethidium bromide used to stain DNA in gel electrophoresis?
| 1. | It increases the DNA’s mobility through the gel. |
| 2. | It condenses the DNA to allow it to enter the gel. |
| 3. | It fluoresces under UV light, allowing visualization of DNA bands. |
| 4. | It cleaves DNA at specific bases, allowing it to be visualized. |
Subtopic: Separation and Isolation of DNA fragments |
92%
From NCERT
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Consider the given two statements:
| I: | Transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). |
| II: | Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools |
68%
From NCERT
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Consider the two statements:
| Assertion (A): | Restriction enzymes produced by bacterial cells do not harm their own DNA |
| Reason (R): | Bacterial DNA are single-stranded molecules. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is True |
Subtopic: Restriction Enzymes - Main Enzymes |
79%
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Plasmids
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | are inherited from one generation to the next. |
| II: | may carry genes that give their host a selective advantage. |
| III: | can render bacteria drug-resistant. |
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Cloning Vector |
80%
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Consider the given two statements
| Assertion (A): | In agar gel electrophoresis, smaller DNA fragments move farther than the larger DNA fragments. |
| Reason (R): | In agar gel electrophoresis, DNA fragments separate according to their size due to the sieving effect of the agarose gel. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is False. |
Subtopic: Separation and Isolation of DNA fragments |
88%
From NCERT
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Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
| II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Restriction Enzymes - Main Enzymes |
From NCERT
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A plasmid is an ideal cloning vector because it:
| 1. | Can only replicate in bacterial cells |
| 2. | Has a single recognition site for all restriction enzymes |
| 3. | Contains an origin of replication, allowing it to replicate independently of the chromosomal DNA |
| 4. | Can incorporate large fragments of DNA without any limit |
Subtopic: Cloning Vector |
93%
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