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Q. No.Consider the given two statements:
Assertion (A):
A selectable marker in a plasmid is necessary for identifying recombinant bacteria.
Reason (R):
Selectable markers help in the replication of plasmids inside the host bacteria.
1.
Both (A) and (R) are True and (R) is the correct explanation of (A).
2.
Both (A) and (R) are True but (R) is not the correct explanation of (A).
3.
(A) is True but (R) is False.
4.
(A) is False but (R) is True.
Subtopic: Selectable Markers |
77%
From NCERT
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A plasmid is an ideal cloning vector because it:
| 1. | Can only replicate in bacterial cells |
| 2. | Has a single recognition site for all restriction enzymes |
| 3. | Contains an origin of replication, allowing it to replicate independently of the chromosomal DNA |
| 4. | Can incorporate large fragments of DNA without any limit |
Subtopic: Cloning Vector |
93%
From NCERT
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Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
| II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Restriction Enzymes - Main Enzymes |
From NCERT
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Why is ethidium bromide used to stain DNA in gel electrophoresis?
| 1. | It increases the DNA’s mobility through the gel. |
| 2. | It condenses the DNA to allow it to enter the gel. |
| 3. | It fluoresces under UV light, allowing visualization of DNA bands. |
| 4. | It cleaves DNA at specific bases, allowing it to be visualized. |
Subtopic: Separation and Isolation of DNA fragments |
92%
From NCERT
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What is a key difference between traditional hybridization techniques and recombinant DNA technology?
| 1. | Traditional hybridization involves sexual reproduction between plants, while recombinant DNA technology involves the chemical synthesis of DNA. |
| 2. | Traditional hybridization can only combine DNA from similar species, while recombinant DNA technology can combine DNA from any species. |
| 3. | Recombinant DNA technology uses live animals, whereas traditional hybridization uses only plant species. |
| 4. | Traditional hybridization is faster than recombinant DNA technology. |
Subtopic: Principles of Biotechnology |
62%
From NCERT
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Restriction Enzymes - Main Enzymes |
57%
From NCERT
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The origin of replication sequence in a plasmid:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | is a sequence from where replication starts |
| II: | is a sequence responsible for controlling the copy number of linked DNA |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Cloning Vector |
96%
From NCERT
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Consider the given two statements:
| I: | Transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). |
| II: | Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools |
68%
From NCERT
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Consider the two statements:
| Assertion (A): | Restriction enzymes produced by bacterial cells do not harm their own DNA |
| Reason (R): | Bacterial DNA are single-stranded molecules. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is True |
Subtopic: Restriction Enzymes - Main Enzymes |
79%
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Plasmids
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | are inherited from one generation to the next. |
| II: | may carry genes that give their host a selective advantage. |
| III: | can render bacteria drug-resistant. |
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Cloning Vector |
80%
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