The main enzyme involved in DNA replication is referred to as DNA-dependent DNA polymerase, since:
1. it uses a DNA template to catalyse the polymerisation of deoxyribonucleotides.
2. it uses a DNA template to catalyse the polymerisation of ribonucleotides.
3. it uses an RNA template to catalyse the polymerisation of deoxyribonucleotides.
4. it uses an RNA template to catalyse the polymerisation of ribonucleotides.

Subtopic:  DNA Replication: I |
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The promoter in a transcription unit:
I: is said to be located towards 3'-end (upstream) of the structural gene (the reference is made with respect to the polarity of template strand).
II: is a DNA sequence that provides binding site for RNA polymerase.
III: defines the template and coding strands.
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic:  Transcription |
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Allelic sequence variation has traditionally been described as a DNA polymorphism if more than one variant (allele) at a locus occurs in human population with a frequency greater than:
1. 0.01 2. 0.05
3. 0.10 4. 1.00
Subtopic:  DNA Fingerprinting |
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The technique of DNA Fingerprinting was initially developed by:
1. Kary Mullis 2. Alec Jeffreys
3. Paul Berg 4. Thomas Cech
Subtopic:  DNA Fingerprinting |
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In DNA fingerprinting involving Southern blot hybridisation using radiolabelled VNTR as a probe, the correct chronology of steps between isolation of DNA [first step] and detection of hybridised DNA fragments by autoradiography [last step] is:
1. separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe
2. digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe
3. separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
4. digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
Subtopic:  DNA Fingerprinting |
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The length of DNA is:
I: usually defined as number of nucleotides (or a pair of nucleotide referred to as base pairs) present in it.
II: is the characteristic of an organism.
 
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic:  The DNA |
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Consider the given two statements:
Statement I: A nitrogenous base is linked to the OH of 1'C pentose sugar through a N-glycosidic linkage to form a nucleoside.
Statement II: When a phosphate group is linked to OH of 5'C of a nucleoside through phosphoester linkage, a corresponding nucleotide (or deoxynucleotide depending upon the type of sugar present) is formed.
 
1. Statement I is correct; Statement II is incorrect
2. Statement I is incorrect; Statement II is incorrect
3. Statement I is correct; Statement II is correct
4. Statement I is incorrect; Statement II is correct
Subtopic:  The DNA |
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In the given diagram:
I: A is 3’-5’ direction.
II: B is hydrogen bond.
III: C is phosphodiester bond.
 
1. Only I and II are correct
2. Only II and III are correct
3. I, II and III are correct
4. I, II, and III are incorrect
Subtopic:  DNA Double Helix |
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When compared to DNA, in RNA:
I: every nucleotide residue has an additional –OH group present at 3'-position in the ribose.
II: 5-methyl uracil is found instead of thymine.
 
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic:  DNA vs RNA as Genetic Material |
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Consider the given two statements:
Assertion (A): If the sequence of bases in one strand of the dsDNA molecule is known then the sequence in other strand can be predicted.
Reason (R): The base pairing in a dsDNA molecule is complementary to each other.
  
1. Both (A) and (R) are True but (R) does not correctly explain (A).
2. (A) is True but (R) is False.
3. (A) is False but (R) is True.
4. Both (A) and (R) are True and (R) correctly explains (A).
Subtopic:  DNA Double Helix |
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