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Q. No.Prior to the work of Oswald Avery, Colin MacLeod and Maclyn McCarty (1933-44), the genetic material was thought to be:
1. a protein
2. a polysaccharide
3. a lipid
4. an RNA
Subtopic: Search for Genetic Material:I | Search for Genetic Material: II |
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Beginning with \(^{15} \text N ^{15} \text N\) DNA [\(^{15} \text N\) is the heavy isotope of nitrogen] and after two rounds of replication in \(^{14} \text N\) medium, E.coli cells will contain:
| 1. | \(\small\mathrm{{25 \%~ { }^{15} {N}^{15} {N} { DNA}; ~50 \%~ { }^{15} {N}^{14} {N} { DNA};~ 25 \%~ { }^{14} {N}^{14} {N} { DNA} }}\) |
| 2. | \(\small\mathrm{{25 \%~ { }^{15} {N}^{14} {N} { DNA}; ~50 \%~ { }^{15} {N}^{15} {N}{ DNA};~25 \%~ { }^{14} {N}^{14} {N} { DNA} }}\) |
| 3. | \(\small\mathrm{50 \%~ { }^{15} {N}^{15} {N} { DNA}; ~50 \%~ { }^{15} {N}^{14} {N} { DNA} }\) |
| 4. | \(\small\mathrm{50 \%~ { }^{15} {N}^{14} {N}{ DNA}; ~50 \%~ { }^{14} {N}^{14} {N} { DNA}}\) |
Subtopic: DNA Replication |
66%
From NCERT
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The main enzyme involved in DNA replication is referred to as DNA-dependent DNA polymerase, since:
| 1. | it uses a DNA template to catalyse the polymerisation of deoxyribonucleotides. |
| 2. | it uses a DNA template to catalyse the polymerisation of ribonucleotides. |
| 3. | it uses an RNA template to catalyse the polymerisation of deoxyribonucleotides. |
| 4. | it uses an RNA template to catalyse the polymerisation of ribonucleotides. |
Subtopic: DNA Replication: I |
89%
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The promoter in a transcription unit:
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | is said to be located towards 3'-end (upstream) of the structural gene (the reference is made with respect to the polarity of template strand). |
| II: | is a DNA sequence that provides binding site for RNA polymerase. |
| III: | defines the template and coding strands. |
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Transcription |
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From NCERT
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Allelic sequence variation has traditionally been described as a DNA polymorphism if more than one variant (allele) at a locus occurs in human population with a frequency greater than:
| 1. | 0.01 | 2. | 0.05 |
| 3. | 0.10 | 4. | 1.00 |
Subtopic: DNA Fingerprinting |
80%
From NCERT
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The technique of DNA Fingerprinting was initially developed by:
| 1. | Kary Mullis | 2. | Alec Jeffreys |
| 3. | Paul Berg | 4. | Thomas Cech |
Subtopic: DNA Fingerprinting |
92%
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In DNA fingerprinting involving Southern blot hybridisation using radiolabelled VNTR as a probe, the correct chronology of steps between isolation of DNA [first step] and detection of hybridised DNA fragments by autoradiography [last step] is:
| 1. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 2. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 3. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
| 4. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
Subtopic: DNA Fingerprinting |
73%
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The length of DNA is:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | usually defined as number of nucleotides (or a pair of nucleotide referred to as base pairs) present in it. |
| II: | is the characteristic of an organism. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: The DNA |
80%
From NCERT
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Consider the given two statements:
| Statement I: | A nitrogenous base is linked to the OH of 1'C pentose sugar through a N-glycosidic linkage to form a nucleoside. |
| Statement II: | When a phosphate group is linked to OH of 5'C of a nucleoside through phosphoester linkage, a corresponding nucleotide (or deoxynucleotide depending upon the type of sugar present) is formed. |
| 1. | Statement I is correct; Statement II is incorrect |
| 2. | Statement I is incorrect; Statement II is incorrect |
| 3. | Statement I is correct; Statement II is correct |
| 4. | Statement I is incorrect; Statement II is correct |
Subtopic: The DNA |
88%
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In the given diagram:
1. Only I and II are correct
2. Only II and III are correct
3. I, II and III are correct
4. I, II, and III are incorrect
| I: | A is 3’-5’ direction. |
| II: | B is hydrogen bond. |
| III: | C is phosphodiester bond. |
1. Only I and II are correct
2. Only II and III are correct
3. I, II and III are correct
4. I, II, and III are incorrect
Subtopic: DNA Double Helix |
66%
From NCERT
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