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Q. No.The technique of DNA Fingerprinting was initially developed by:
1.
Kary Mullis
2.
Alec Jeffreys
3.
Paul Berg
4.
Thomas Cech
Subtopic: DNA Fingerprinting |
92%
From NCERT
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In DNA fingerprinting involving Southern blot hybridisation using radiolabelled VNTR as a probe, the correct chronology of steps between isolation of DNA [first step] and detection of hybridised DNA fragments by autoradiography [last step] is:
| 1. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 2. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 3. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
| 4. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
Subtopic: DNA Fingerprinting |
73%
From NCERT
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The length of DNA is:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | usually defined as number of nucleotides (or a pair of nucleotide referred to as base pairs) present in it. |
| II: | is the characteristic of an organism. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: The DNA |
80%
From NCERT
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Consider the given two statements:
| Statement I: | A nitrogenous base is linked to the OH of 1'C pentose sugar through a N-glycosidic linkage to form a nucleoside. |
| Statement II: | When a phosphate group is linked to OH of 5'C of a nucleoside through phosphoester linkage, a corresponding nucleotide (or deoxynucleotide depending upon the type of sugar present) is formed. |
| 1. | Statement I is correct; Statement II is incorrect |
| 2. | Statement I is incorrect; Statement II is incorrect |
| 3. | Statement I is correct; Statement II is correct |
| 4. | Statement I is incorrect; Statement II is correct |
Subtopic: The DNA |
88%
From NCERT
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In the given diagram:
1. Only I and II are correct
2. Only II and III are correct
3. I, II and III are correct
4. I, II, and III are incorrect
| I: | A is 3’-5’ direction. |
| II: | B is hydrogen bond. |
| III: | C is phosphodiester bond. |
1. Only I and II are correct
2. Only II and III are correct
3. I, II and III are correct
4. I, II, and III are incorrect
Subtopic: DNA Double Helix |
66%
From NCERT
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When compared to DNA, in RNA:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | every nucleotide residue has an additional –OH group present at 3'-position in the ribose. |
| II: | 5-methyl uracil is found instead of thymine. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: DNA vs RNA as Genetic Material |
From NCERT
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Consider the given two statements:
| Assertion (A): | If the sequence of bases in one strand of the dsDNA molecule is known then the sequence in other strand can be predicted. |
| Reason (R): | The base pairing in a dsDNA molecule is complementary to each other. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is True but (R) is False. |
| 3. | (A) is False but (R) is True. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: DNA Double Helix |
86%
From NCERT
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A hypothetical dsDNA of 100 bp length molecule was found to be composed of 40 Adenine residues. The total number of hydrogen bonds in all base pairs of this molecule is expected to be:
| 1. | 100 | 2. | 130 |
| 3. | 260 | 4. | 300 |
Subtopic: DNA Double Helix |
79%
From NCERT
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Consider the given two statements:
| Assertion (A): | Viruses having RNA genome and having shorter life span mutate and evolve faster. |
| Reason (R): | RNA being unstable, mutates at a faster rate. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is True but (R) is False. |
| 3. | (A) is False but (R) is True. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: DNA vs RNA as Genetic Material |
84%
From NCERT
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Consider the given two statements:
1. Both I and II are correct
2. Only II is correct
3. Only I is correct
4. Both I and II are incorrect
| Statement I: | A transcription unit in DNA is the structural gene that is flanked by the promoter and the terminator. |
| Statement II: | A translational unit in mRNA is the sequence of RNA that is flanked by the start codon (AUG) and the stop codon |
1. Both I and II are correct
2. Only II is correct
3. Only I is correct
4. Both I and II are incorrect
Subtopic: Transcription:II |
85%
From NCERT
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