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Q. No.In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
1.
Pvu I
2.
BamH I
3.
Pst I
4.
Pvu II
Subtopic: Selectable Markers: III |
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In a plasmid cloning vector what is responsible for controlling the copy number of the linked DNA?
1. Ori
2. MCS
3. Selectable marker
4. Promoter
1. Ori
2. MCS
3. Selectable marker
4. Promoter
Subtopic: Tools: Vectors: I |
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Consider the given two statements:
| Assertion (A): | The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. |
| Reason (R): | The normal E. coli cells do not carry resistance against any of these antibiotics. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Selectable Markers: I |
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In agarose gel electrophoresis the separated DNA fragments can be visualized only after staining the DNA with a compound known as:
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
1. Polyethylene glycol
2. Janus Green
3. DEC
4. Ethidium bromide
Subtopic: Separation and Isolation of DNA fragments |
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Biotechnology has been defined as ‘the integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and services, by:
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
1. EFB
2. American Chemical Society
3. GEAC
4. NCBI
Subtopic: Introduction & History: I |
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Identify the correct statements:
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Herbert Boyer performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. |
| II: | Stanley Cohen developed a method of removing plasmids from the bacterial cell and then reinserting them in other bacterial cells. |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Introduction & History: I |
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Maintenance of sterile ambience in chemical engineering processes to enable growth of only the desired microbe/eukaryotic cell in large quantities for the manufacture of biotechnological products like antibiotics, vaccines, enzymes, etc is called as:
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
1. Genetic engineering
2. Bioprocess engineering
3. Gene technology
4. Biopharming
Subtopic: Introduction & History: II |
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Restriction enzymes have been isolated from:
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
I: Bacteria
II: Yeasts
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Restriction Enzymes - Main Enzymes |
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What can be the natural function of a restriction enzyme?
| 1. | To remove introns from hnRNA |
| 2. | To cut dsRNA resulting in the formation of miRNA |
| 3. | To cut foreign DNA |
| 4. | To join exons after removal of introns from a transcript |
Subtopic: Introduction & History: II |
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Bacterial cells add methyl groups to their DNA in order to:
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
Subtopic: Restriction Enzymes - Main Enzymes |
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