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Q. No.In the processes of Recombinant DNA Technology, downstream processing:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | occurs after the completion of the biosynthetic stage. |
| II: | involves processes that make products ready for marketing as a finished product. |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Bioreactors/Downstream Processing |
92%
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
90%
From NCERT
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Consider the given two statements:
| Assertion (A): | In most recombinant technologies, there is a need for the recombinant DNA to be expressed. |
| Reason (R): | In most recombinant technologies, the ultimate aim is to produce a desirable protein. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Obtaining Copy of Gene from Donor DNA |
77%
From NCERT
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Consider the given two statements:
| Assertion (A): | The most commonly used bioreactors for biosynthesis are of stirring type. |
| Reason (R): | A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Bioreactors/Downstream Processing |
55%
From NCERT
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
| 1. | 1000 copies | 2. | One lakh copies |
| 3. | One million copies | 4. | One billion copies |
Subtopic: Polymerase Chain Reaction: PCR |
86%
From NCERT
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The source of thermostable DNA polymerase used in Polymerase Chain Reaction is:
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
Subtopic: Polymerase Chain Reaction: PCR |
92%
From NCERT
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
81%
From NCERT
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic: Process of Biotech |
79%
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic: Cloning Vector |
84%
From NCERT
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In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
| 1. | Pvu I | 2. | BamH I |
| 3. | Pst I | 4. | Pvu II |
Subtopic: Selectable Markers: III |
80%
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