Consider the given two statements:
Assertion (A):   In most recombinant technologies, there is a need for the recombinant DNA to be expressed.
Reason (R): In most recombinant technologies, the ultimate aim is to produce a desirable protein.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. (A) is True; (R) is False
3. (A) is False; (R) is False
4. Both (A) and (R) are True but (R) does not correctly explain (A).

Subtopic:  Obtaining Copy of Gene from Donor DNA |
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Consider the given two statements:
Assertion (A):  The most commonly used bioreactors for biosynthesis are of stirring type.
Reason (R): A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. (A) is True; (R) is False
3. (A) is False; (R) is False
4. Both (A) and (R) are True but (R) does not correctly explain (A).
Subtopic:  Bioreactors/Downstream Processing |
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
1. 1000 copies 2. One lakh copies
3. One million copies 4. One billion copies
Subtopic:  Polymerase Chain Reaction: PCR |
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The source of thermostable DNA polymerase used in Polymerase Chain Reaction is:
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
Subtopic:  Polymerase Chain Reaction: PCR |
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
1. ligase is added.
2. the temperature of the apparatus is increased to 95 degree Celsius.
3. a suitable host cell is incubated with the fragments.
4. primers are added for annealing to DNA fragments.
Subtopic:  Process of Biotech |
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic:  Process of Biotech |
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The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens:
1. is unique as it is single stranded DNA molecule.
2. is not pathogenic to plants.
3. has now been modified into a cloning vector.
4. is frequently used to transform monocots.
Subtopic:  Cloning Vector |
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In E. coli cloning vector pBR322, restriction site of which of the following restriction enzymes does not lie within a gene for antibiotic resistance?
1. Pvu I 2. BamH I
3. Pst I 4. Pvu II
Subtopic:  Selectable Markers: III |
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In a plasmid cloning vector what is responsible for controlling the copy number of the linked DNA?
1. Ori
2. MCS
3. Selectable marker
4. Promoter
 
Subtopic:  Tools: Vectors: I |
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Consider the given two statements:
Assertion (A): The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
Reason (R):  The normal E. coli cells do not carry resistance against any of these antibiotics.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. (A) is True; (R) is False
3. (A) is False; (R) is False
4. Both (A) and (R) are True but (R) does not correctly explain (A).
Subtopic:  Selectable Markers: I |
 54%
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