Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
2. | The enzyme cuts DNA molecules at an identified position within the DNA |
3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. | Eco Rl | 2. | taq polymerase |
3. | polymerase lll | 4. | ligase |
1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
2. | The process of extraction of separated DNA strands from gel is called elution. |
3. | The separated DNA fragments are stained by using ethidium bromide. |
4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Biolistics (gene-gun) is suitable for:
1. | Transformation of plant cells |
2. | Constructing recombinant DNA by joining with vectors |
3. | DNA fingerprinting |
4. | Disarming pathogen vectors |
Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | Both (A) and (R) are True and (R) does not correctly explain (A). |
3. | (A) is True, (R) is False. |
4. | (A) is False, (R) is True. |
In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Assertion (A): | \(\beta\)-galactosidase coding sequence act as a selectable marker. |
Reason (R): | Enzyme galactosidase converts galactose into lactose. |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
3. | (A) is True but (R) is False. |
4. | (A) is False but (R) is True. |
1. | Polysaccharides | 2. | RNA |
3. | DNA | 4. | Histones |