Q. No.DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments are cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is referred to as :
1. restriction map 2. RFLP profile 3. F' factor 4. library
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
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A key feature of insertional mutagenesis for the identification of plasmids containing recombinant DNA is:
| 1. | the production of nutritional auxotrophs. |
| 2. | the DNA sequencing of recombinant plasmids. |
| 3. | the production of restriction endonuclease maps of recombinant plasmids. |
| 4. | the disruption of a gene on the plasmid by the inserted recombinant DNA. |
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
1. electroporation
2. liposome transfer
3. microinjection
4. particle bombardment
What type of probe would you use for a Western blot experiment?
| 1. | A known DNA sequence. | 2. | An RNA molecule. |
| 3. | A purified protein. | 4. | An antibody. |
If you are using a vector with lacZ as a screenable marker, which of the following bacterial strains would you want to use as host cells?
1. lacO-
2. lacI-
3. lacZ-
4. Any of these would work.
Which of the enzymes whose recognition sites are shown here would produce sticky ends compatible with BstAUI (5'-G|GTACC-3')?
1. Bcl I (5'-T|GATCA-3')
2. Sun I (5'-C|GTACG-3')
3. Hind III (5'-A|AGCTT-3')
4. Kpn I (5'-GGTAC|C-3')
Which of the following would NOT be a useful selectable marker?
| 1. | A gene encoding a protein that degrades the antibiotic ampicillin. |
| 2. | A gene encoding a protein that allows the cell to synthesize histidine. |
| 3. | A gene encoding a protein that is an essential structural component of the cell. |
| 4. | All of these are useful selectable markers. |
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Which of the following primers would allow copying of the single-stranded DNA sequence 5' ATGCCTAGGTC?
| 1. | 5' TACGG | 2. | 5' CTGGA |
| 3. | 5' GACCT | 4. | 5' GGCAT |
Hybridization of mRNA with DNA can reveal which segment of the DNA are introns and which segment are exons. When the magnification is high enough to see the molecule, the introns appear as
1. Single stranded loops in a double stranded molecule
2. Separate fragments
3. Triple stranded nucleic acid molecules
4. Barr bodies
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