Q. No.Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?
1.
restriction endonuclease - production of DNA fragments for gene cloning.
2.
DNA polymerase - copies DNA sequences to create primers in the polymerase chain reaction.
3.
reverse transcriptase - production of cDNA from mRNA.
4.
electrophoresis - RLFP analysis.
What process causes shortening of telomeres during cell division?
1. DNA synthesis.
2. Exonuclease removal of primers.
3. Ligation of DNA fragments.
4. Telomerase activity.
The DNA profiles below represent four different individuals. Which of the following statements is consistent with the results?
1. B is the child of A and C.
2. C is the child of A and B.
3. D is the child of B and C.
4. A is the child of B and C.
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Dideoxyribonucleotide chain-termination is a method of
1. cloning DNA.
2. sequencing DNA.
3. digesting DNA.
4. synthesizing DNA.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
| I. | Transform bacteria with recombinant DNA molecule. |
| II. | Cut the plasmid DNA using restriction enzymes. |
| III. | Extract plasmid DNA from bacterial cells. |
| IV. | Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. |
| V. | Use ligase to seal plasmid DNA to non-plasmid DNA. |
1. I, II, IV, III, V
2. II, III, V, IV, I
3. III, II, IV, V, I
4. III, IV, V, I, II
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The "Southern" technique involves:
| 1. | the detection of DNA fragments on membranes by specific radioactive antibodies. |
| 2. | the detection of proteins on membranes using a radioactive DNA probe. |
| 3. | the detection of DNA fragments on membranes by a radioactive DNA probe. |
| 4. | the detection of proteins on membranes using specific radioactive antibodies. |
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A major advantage of using YAC as a cloning vector over the plasmids is that :
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
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Heat-killed S form Streptococcus extract treated with proteases is mixed with live R form Streptococcus cells and injected into living mice. What is most likely to happen?
| 1. | The mice would live. |
| 2. | The mice would die. |
| 3. | The mice's blood would have dead R cells. |
| 4. | A new strain of Streptococcus will be present in mice. |
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The addition of deoxyribonucleotides by DNA polymerase during DNA synthesis is an endergonic process. The source of energy used is:
| 1. | ATP |
| 2. | Energy from photons of light |
| 3. | Entropy created from protein digestion |
| 4. | The nucleotides themselves |
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It is believed that all organisms have arisen from a single distant ancestor. The strongest evidence for this would be:
| 1. | DNA stores genetic information in all organisms. |
| 2. | The genetic code is universally a triplet code. |
| 3. | The amino acids specified by particular triplets are almost always identical between any two organisms. |
| 4. | The genetic code is degenerate but unambiguous. |
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