If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

1.      5’TAACGGGATC 3’

2.      3’TAACGGGATC 5’

3.      5’ATTGCCCTAG 3’

4.     3’ATTGCCCTAG 5’

If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

1.      10240

2.      1024

3.      512

4.     5120

What is the final step of PCR?

1.      Extension

2.      Annealing

3.      Denaturation

4.     Extraction

After 30 cycles of PCR, how many times DNA will be amplified?

1.      Thousand times

2.      Million times

3.      Billion times

4.     Hundreds times

Use of primers, occurs in which step of PCR?

1.     Annealing

2.     Extension

3.     Denaturation

4.     Both extension and Annealing

The ultimate aim of maximum recombinant DNA technologies is

1.   To multiply DNA

2.   To express DNA

3.   To multiply mRNA

4.   To express protein

Which of the following processes is not associated with the protein produced by recombinant DNA?

1.      Restriction Digestion

2.      Expression

3.      Optimization

4.     Purification

What does continuous culture mean?

1. Where DNA and protein getting expressed continuously

2. Where used medium is drained out and the fresh medium is added from another side

3. Where cells are producing protein for one week continuously

4. Where the production of recombinant DNA is continuing without interference.

Continuous culture has

1.      Cells in their physically active phase

2.      Lag phase

3.      Both A and B

4.     Exponential phase

Continuous culture leads to

1. Smaller biomass

2. Higher yields of desired proteins

3. Both 1 and 2

4. Higher yields of recombinant DNA