If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

If I want to check the expression of my gene, then I should use

Selectable marker helps

The normal E. coli cells have

For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

1.      Non- recombinant

2.      Recombinant

3.      Transformants

4.     Non-transformants

Insertional inactivation results in

1.      Non-recombinants

2.      Recombinants

3.      Transformants

4.     Non- transformants

Which of the following options is correct about Agrobacterium tumifaciens?

In order to make the host cell competent

Divalent cations such as calcium are used-

1.      The divalent ions have to be in a specific concentration

2.      They cause the DNA uptake by the cell

3.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

4.     All of the above