The normal E. coli cells have

Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning

Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

1.      Non- recombinant

2.      Recombinant

3.      Transformants

4.     Non-transformants

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Selectable marker helps

If I want to check the expression of my gene, then I should use