Arrange the following enzymes/ processes in the order of their use for a certain experiment of genetic engineering:

1) ligase,
2) restriction enzyme
3)desired gene
4) Transformation
5) Expression

1.      3-2-1-4-5

2.      1-2-4-5-3

3.      3-1-4-5-2

4.     2-5-4-3-1

What does cloning mean?

1.      The ability to multiply the desired gene in the host

2.      The ability to multiply the vector in the host

3.      The ability to multiply the antibiotic resistance gene in the host

4.     The ability to multiple Ori in the host

Which of the following is not a tool of RDT?

1.      PCR

2.      Restriction enzymes

3.      Vectors

4.     Host organisms

Which of the following is a tool of RDT?

1.      PCR

2.      Gel Electrophoresis

3.      Cloning

4.     Restriction enzyme

In the year 1963, the two enzymes responsible for inhibiting the growth of bacteriophage in E. coli were isolated. One of them was

1.      Ligase

2.      Polymerase

3.      Molecular scissor

4.     Antibiotic resistance enzyme

Hind II

1.      First restriction endonuclease

2.      Was characterized 7 years later

3.      Specific sequence of 5 base pairs

4.     All of these

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1.      700; 350

2.      900; 230

3.      600; 270

4.     550; 210

Which of the following is correct representation of the name of enzyme?

1.      ECORI

2.      EcoRI

3.      ECoRI

4.     ECoR1

EcoRI comes from

1.      Escherichia coli RY13

2.      Escherichia coli RY16

3.      Escherichia coli RY19

4.     Escherichia coli RY12

In EcoRI, here I stands for

1.      Order in which strains are discovered

2.      Order in which enzymes are isolated

3.      Order in which enzymes are isolated from that strain

4.      Order in which strains are reported in microbiology