Elution is:

1. Separating the restricted DNA fragments on agarose gel.

2. Staining the separate DNA fragments with ethidium bromide

3. cutting out of the separated band of DNA from the agarose gel and extracting them from the gel piece.

4. constructing rDNA by joining the purified DNA fragments to the cloning vector.

When isolating the pure DNA from a bacterial cell, the cell should not be treated with:

1. lysozyme

2. proteases

3. ribonuclease

4. deoxyribonuclease

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

cDNA probes are copied from the messenger RNA molecules with the help of

1. restriction enzymes

2. reverse transcriptase

3. DNA polymerase

4. adenosine deaminase.

Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?

(1) Lysozyme, lipases, trypsin

(2) Chitinase, cellulase, lysozyme

(3) Chitinase, cellulase, trypsin

(4) Trypsin, lipases, cellulase