| A. | Digestion of cell walls. |
| B. | Isolation of naked protoplasts. |
| C. | Fusion of protoplasts to get hybrid protoplast. |
| D. | Isolation of single cells from two different varieties of plants. |
| E. | Growing of hybrid protoplast to form a new plant. |
| 1. | E, A, B, C, D | 2. | D, A, B, C, E |
| 3. | E, B, A, D, C | 4. | D, B, A, E, C |
| 1. | Micropropagation |
| 2. | Differentiation |
| 3. | Somatic hybridization |
| 4. | Totipotency |
| 1. | Somatic embryos | 2. | Protoplasts |
| 3. | Pollens | 4. | Callus |
Match List - I with List - II
| List - I | List - II |
| (a) Protoplast fusion | (i) Totipotency |
| (b) Plant tissue culture | (ii) Pomato |
| (c) Meristem culture | (iii) Somaclones |
| (d) Micropropagation | (iv) Virus free plants |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (iii) | (iv) | (i) | (ii) |
| 2. | (iv) | (iii) | (ii) | (i) |
| 3. | (iii) | (iv) | (ii) | (i) |
| 4. | (ii) | (i) | (iv) | (iii) |
To obtain virus-free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken?
| 1. | Apical meristem only |
| 2. | Palisade parenchyma |
| 3. | Both apical and axillary meristems |
| 4. | Epidermis only |
Which part would be most suitable for raising virus-free plants for micro propagation?
1. Bark
2. Vascular tissue
3. Meristem
4. Node
Somaclones are obtained by:
1. tissue culture
2. plant breeding
3. irradiation
4. genetic engineering
In order to obtain virus-free plants through tissue culture the best method is:
1. protoplast culture
2. embryo rescue
3. anther culture
4. meristem culture
The technique of obtaining a large number of plantlets by tissue culture method is called:
1. Organ culture
2. Micropropagation
3. Micropropagation
4. Plantlet culture
Cellular totipotency is demonstrated by:
| 1. | Only gymnosperm cells | 2. | All plant cells |
| 3. | All eukaryotic cells | 4. | Only bacterial cells |