1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
2. | The process of extraction of separated DNA strands from gel is called elution. |
3. | The separated DNA fragments are stained by using ethidium bromide. |
4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
(a) | Agarose is a natural polymer obtained from sea-weed. |
(b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
(c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
(d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
1. | (a) and (b) only | 2. | (a), (b) and (c) only |
3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
1. | Dark red bands | 2. | Bright blue bands |
3. | Yellow bands | 4. | Bright orange bands |
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1. | Histones | 2. | Polysaccharides |
3. | RNA | 4. | DNA |
Match the following techniques or instruments with their usage:
(a) | Bioreactor | (i) | Separation of DNA fragments |
(b) | Electrophoresis | (ii) | Production of large quantities of products |
(c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
(d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Spooling is:
1. | Amplification of DNA |
2. | Cutting of separated DNA bands from the agarose gel |
3. | Transfer of separated DNA fragments to synthetic membranes |
4. | Collection of isolated DNA |
Select the correct statement from the following:
1. | Gel electrophoresis is used for the amplification of a DNA segment. |
2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
4. | PCR is used for the isolation and separation of genes of interest. |