In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
Statement I: | Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. |
Statement II: | Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site. |
Given below are two statements: One is labeled as Assertion (A) and the other is labeled as Reason (R)
Assertion (A): | Restriction enzyme is a type of endonuclease |
Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
1. | Both (A) and (R) are true and (R) correctly explains (A). |
2. | Both (A) and (R) are true and (R) does not correctly explain (A). |
3. | (A) is true, (R) is false. |
4. | (A) is false, (R) is true. |
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
Which of the following restriction enzymes produces blunt ends?
1. Sal l
2. Eco RV
3. Xho
4. Hind lll
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases