Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduces DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperatures
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents.
1. | A - Denaturation at a temperature of about 50ºC |
2. | C - Extension in the presence of heat-stable DNA polymerase |
3. | A - Annealing with two sets of primers |
4. | B - Denaturation at a temperature of about 98ºC separating the two DNA strands |