A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
1. Eco Rl
2. taq polymerase
3. polymerase lll
4. ligase
Which of the following restriction enzymes produces blunt ends?
1. Sal l
2. Eco RV
3. Xho
4. Hind lll
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
1. | Vector | 2. | Plasmid |
3. | Structural gene | 4. | Selectable marker |
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
1. | The smaller the fragment size, the farther it moves |
2. | Positively charged fragments move to farther end |
3. | Negatively charged fragments do not move |
4. | The larger the fragment size, the farther it moves |
The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid