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Q. No.Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
Assertion (A):
Restriction enzyme is a type of endonuclease
Reason (R):
Restriction enzyme cuts the two stands of DNA at specific positions within the DNA
In the light of the above statements, choose the correct answer from the options given below:
1.
(A) is True but (R) is False.
2.
(A) is False but (R) is True.
3.
Both (A) and (R) are True and (R) is the correct explanation of (A).
4.
Both (A) and (R) are True but (R) is not the correct explanation of (A).
In the light of the above statements, choose the correct answer from the options given below:
Subtopic: Restriction Enzymes - Main Enzymes |
73%
Level 2: 60%+
NEET - 2022
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Tools |
60%
Level 2: 60%+
NEET - 2022
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
87%
Level 1: 80%+
NEET - 2022
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Match List I with List II:
Choose the correct answer from the options given below:
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
| List I | List II |
| A. Chloramphenicol | I. Join the cut ends of DNA molecule |
| B. Ethidium bromide | II. Makes bacterial cell competent to take up DNA |
| C. DNA ligase | III. Selectable marker |
| D. Calcium | IV. Agarose gel staining |
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
Subtopic: General Design of an rDNA experiment |
92%
Level 1: 80%+
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Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: General Design of an rDNA experiment |
79%
Level 2: 60%+
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Match List-I with List-II:
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
Subtopic: Transforming Plant & Animal Cell |
92%
Level 1: 80%+
NEET - 2022
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Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Separation and Isolation of DNA fragments |
83%
Level 1: 80%+
NEET - 2022
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Read the following statements and choose the correct option.
| Statement (A): | In gel electrophoresis, the most commonly used matrix is agar. |
| Statement (B): | Agar is a natural polymer extracted from sea weeds. |
| 1. | Both statements (A) and (B) are correct |
| 2. | Both statements (A) and (B) are incorrect |
| 3. | Only statement (A) is correct |
| 4. | Only statement (B) is correct |
Subtopic: Separation and Isolation of DNA fragments |
Level 4: Below 35%
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How many statements given below are correct with respect to plasmids?
Select the correct option.
1. Three
2. Four
3. Two
4. One
| a. | They float freely in the cytoplasm of certain bacterial cells. |
| b. | They have the ability to replicate, independent of the genomic DNA. |
| c. | Herbert Boyer developed a method of removing and reinserting them in the cells. |
| d. | They are circular ssDNA molecules. |
1. Three
2. Four
3. Two
4. One
Subtopic: Introduction & History |
Level 3: 35%-60%
Hints
Which of the following can act as selectable markers in a cloning vector?
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
Subtopic: Cloning Vector |
86%
Level 1: 80%+
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