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Q. No.
In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
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Polymerase chain reaction (PCR) amplifies DNA following the equation.
| 1. | \(2n+1\) | 2. | \(2N^2\) |
| 3. | \(N^2\) | 4. | \(2^n\) |
Subtopic: Polymerase Chain Reaction: PCR |
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Identify the part of a bio-reactor which is used as a foam braker from the given figure.
| 1. | D | 2. | C |
| 3. | A | 4. | B |
Subtopic: Bioreactors/Downstream Processing |
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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method:
Given below are two statements about this method:
| Statement I: | The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. |
| Statement II: | The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. |
| 1. | Statement I is correct; Statement II is incorrect |
| 2. | Statement I is incorrect; Statement II is correct |
| 3. | Both Statement I and Statement II are correct |
| 4. | Both Statement I and Statement II are incorrect |
Subtopic: Selection of Recombinant Transformants |
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Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. C and D only
4. A and B only
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. C and D only
4. A and B only
Subtopic: Tools |
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Given below are two statements :
In the light of the above statements, choose the most appropriate answer from the options given below :
| Statement I : | The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. |
| Statement II : | Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. |
| 1. | Statement I is correct but statement II is incorrect |
| 2. | Statement I is incorrect but statement II is correct |
| 3. | Both statement I and statement II are correct |
| 4. | Both statement I and statement II are incorrect |
Subtopic: Restriction Enzymes - Main Enzymes |
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
| 1. | D and E only | 2. | B and C only |
| 3. | A and E only | 4. | A and B only |
Subtopic: Principles of Biotechnology |
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
| 1. | 6 bp | 2. | 4 bp |
| 3. | 10 bp | 4. | 8 bp |
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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The " Ti plasmid " of Agrobacterium tumefaciens stands for
| 1. | Tumor independent plasmid |
| 2. | Tumor inducing plasmid |
| 3. | Temperature independent plasmid |
| 4. | Tumour inhibiting plasmid |
Subtopic: Tools: Vectors: I |
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Which of the following statements is incorrect?
| 1. | Most commonly used bio-reactors are of stirring type. |
| 2. | Bio-reactors are used to produce small scale bacterial cultures. |
| 3. | Bio-reactors have an agitator system, an oxygen delivery system and foam control system. |
| 4. | A bio-reactor provides optimal growth conditions for achieving the desired product. |
Subtopic: Bioreactors/Downstream Processing |
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