| A. | Digestion of cell walls. |
| B. | Isolation of naked protoplasts. |
| C. | Fusion of protoplasts to get hybrid protoplast. |
| D. | Isolation of single cells from two different varieties of plants. |
| E. | Growing of hybrid protoplast to form a new plant. |
| 1. | E, A, B, C, D | 2. | D, A, B, C, E |
| 3. | E, B, A, D, C | 4. | D, B, A, E, C |
| 1. | Alzheimer's disease | 2. | Emphysema |
| 3. | Rheumatoid arthritis | 4. | Cystic fibrosis |
| 1. | Virus | 2. | Phage |
| 3. | Bacterium | 4. | Yeast |
| 1. | Complementary tRNA | 2. | Non-complementary ssRNA |
| 3. | Complementary dsRNA | 4. | Inhibitory ssRNA |
| Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. |
| Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. |
| 1. | Micropropagation |
| 2. | Differentiation |
| 3. | Somatic hybridization |
| 4. | Totipotency |
| 1. | Somatic embryos | 2. | Protoplasts |
| 3. | Pollens | 4. | Callus |
| List-I | List-II | ||
| A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm |
| B. | Cry IAb | II. | ADA deficiency |
| C. | Cry IAc | III. | Emphysema |
| D. | Enzyme replacement therapy | IV. | Corn borer |
| Statement I: | Two DNA sequences were prepared corresponding to A and B, chains of human insulin and were introduced in the plasmids of Agrobacterium to produce insulin chain. |
| Statement II: | Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. |