1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
Stirred-tank bioreactors have been designed for:
1. Addition of preservatives to the product
2. Purification of the product
3. Ensuring anaerobic conditions in the culture vessel
4. Availability of oxygen throughout the process
1. | cutting of DNA into fragments |
2. | separation of DNA fragments according to their size |
3. | construction of recombinant DNA by joining with cloning vectors |
4. | isolation of DNA molecule |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
2. | The enzyme cuts DNA molecules at an identified position within the DNA |
3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol
3. Chilled ethanol
4. Methanol at room temperature
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
1. | Bioreactor | 2. | BOD incubator |
3. | Sludge digester | 4. | Industrial oven |
For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
1. Silver or platinum
2. Platinum or zinc
3. Silicon or platinum
4. Gold or tungsten