- 1(current)
Q. No.
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Read the following statements and choose the correct option.
| Statement (A): | In gel electrophoresis, the most commonly used matrix is agar. |
| Statement (B): | Agar is a natural polymer extracted from sea weeds. |
| 1. | Both statements (A) and (B) are correct |
| 2. | Both statements (A) and (B) are incorrect |
| 3. | Only statement (A) is correct |
| 4. | Only statement (B) is correct |
Subtopic: Â Separation and Isolation of DNA fragments |
Level 4: Below 35%
Hints
In agarose gel electrophoresis, DNA molecules are separated on the basis of
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
Subtopic: Â Separation and Isolation of DNA fragments |
 81%
Level 1: 80%+
Hints
| Statement I: | Ethidium bromide-stained DNA can be seen under exposure to UV light when DNA molecules are separated by agarose gel electrophoresis. |
| Statement II: | Ethidium bromide-stained DNA can be cut out and extracted from the agarose gel piece. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | Both Statement I and Statement II are correct. |
| 2. | Both Statement I and Statement II are incorrect. |
| 3. | Statement I is correct but Statement II is incorrect. |
| 4. | Statement I is incorrect but Statement II is correct. |
Subtopic: Â Separation and Isolation of DNA fragments |
 89%
Level 1: 80%+
Hints
| Assertion(A): | Chitinase is used for treating the cells of yeast to assist DNA extraction. |
| Reason(R): | Yeast cell wall is made of chitin. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Â Separation and Isolation of DNA fragments |
 78%
Level 2: 60%+
Hints
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Assertion(A): While loading the DNA fragment into the wells of agarose gel, a blue dye methylene blue is mixed.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â Separation and Isolation of DNA fragments |
Level 4: Below 35%
Hints
Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Â Separation and Isolation of DNA fragments |
 83%
Level 1: 80%+
NEET - 2022
Hints
Read the following statements and choose the correct option:
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
| Statement A: | Agarose gel electrophoresis is employed to separate the fragments of DNA obtained after restriction enzyme digestion. |
| Statement B: | DNA is a negatively charged molecule and moves toward the cathode. |
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Â Separation and Isolation of DNA fragments |
 81%
Level 1: 80%+
Hints
- 1(current)
Q. No.
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