Column-I | Column -II | ||
a. | Biosynthetic stage | (i) | Increased surface area for oxygen transfer |
b. | Downstream processing | (ii) | Large-scale production in the fermenter |
c. | Sparged stirred-tank bioreactor |
(iii) | With foam breaker and flat bladed impeller |
d. | Simple stirred-tank bioreactor |
(iv) | Separation and purification |
Statement A: | Wine making as well as antigen production in recombinant organisms are included in biotechnology |
Statement B: | Biotechnology is a very wide area which deals with techniques of using a natural organism (or its parts) as well as genetic engineering |
Statement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
Column I | Column II | ||
a. | Aniline blue | (i) | Used to stain DNA in agarose gel electrophoresis |
b. | Bromophenol blue | (ii) | stains chromosomes |
c. | Ethidium bromide | (iii) | Tracking dye |
d. | Acetocarmine | (iv) | Used to stain collagen |
Statement A: | Agarose gel electrophoresis is employed to separate the fragments of DNA obtained after restriction enzyme digestion. |
Statement B: | DNA is a negatively charged molecule and moves toward the cathode. |
Assertion (A): | In vitro fertilisation, leading to a 'test-tube' baby, is included under the umbrella of biotechnology |
Reason (R): | The definition of biotechnology, given by EFB encompasses only modern molecular biotechnology view |
1. | (A) is true but (R) is false |
2. | (A) is false but (R) is true |
3. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
4. | Both (A) and (R) are true but (R) is the not the correct explanation of (A) |
Statement A: | Plasmid is a double stranded structure. |
Statement B: | Plasmid is extra chromosomal genetic material in bacteria. |