A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
1. | Denaturation | 2. | Ligation |
3. | Annealing | 4. | Extension |
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
1. | it will lead to the lysis of host cells. |
2. | it will be able to produce a novel protein with dual abilities. |
3. | it will not be able to confer ampicillin resistance to the host cell. |
4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
1. | Histones | 2. | Polysaccharides |
3. | RNA | 4. | DNA |
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
1. Extension, Denaturation, Annealing
2. Annealing, Denaturation, Extension
3. Denaturation, Annealing, Extension
4. Denaturation, Extension, Annealing
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
1. | Dark red bands | 2. | Bright blue bands |
3. | Yellow bands | 4. | Bright orange bands |
In Lederberg's replica plating experiment, what shall be used to obtain streptomycin resistant strain?
1. Minimal medium and streptomycin
2. Complete medium and streptomycin
3. Only minimal medium
4. Only complete medium
Manipulation of DNA in genetic engineering became possible due to the discovery of:
1. Restriction endonuclease
2. DNA ligase
3. Transcriptase
4. Primase
Which of the following enzymes are used to join bits of DNA:
1. Ligase
2. Primase
3. DNA polymerase
4. Endonuclease
Restriction endonucleases:
1. | Are used in genetic engineering for ligating two DNA molecules. |
2. | Are used for in vitro DNA synthesis. |
3. | Are synthesized by bacteria as part of their defense mechanism. |
4. | Are present in mammalian cells for the degradation of DNA when the cell dies. |