If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except
1. Ampicillin rich medium
2. Tetracycline rich medium
3. X-gal rich medium
4. Nutrient medium without antibiotics
If any protein-encoding gene is expressed in a heterologous host, it is termed as
1. Recombinant protein
2. Single-cell protein
3. Humulin
4. Transposon
Read the following statements
Statement-A : Restriction enzymes are obtained only from eukaryotes.
Statement-B : The first discovered restriction endonuclease was EcoRI.
Choose the correct option.
(1) Both statements are correct
(2) Both statements are incorrect
(3) Statement A is correct but B is incorrect
(4) Statement B is correct but A is incorrect
Genetically engineered plants are generated in laboratory by altering their genetic makeup. This is done by adding one or more genes to a plant’s genome by
(1) Biolistic method or Agrobacterium tumefaciens mediated transformation
(2) DNA injection or heat shock method
(3) Fusing liposomes to intact plant cells
(4) Bacillus thuringiensis mediated transformation
The enzyme known as molecular scissors in genetic engineering is
1. DNA ligase
2. Transferases
3. DNA polymerase
4. Restriction endonuclease
During the process of gel electrophoresis, DNA moves towards the __ of the electrophoretic chamber as the charge of DNA is __.
Choose the option that fills the blanks correctly.
A B
1. Cathode Positive
2. Cathode Negative
3. Anode Positive
4. Anode Negative
Downstream processing does not involve
1. Biosynthesis
2. Separation
3. Purification
4. Centrifugation
All the following are transformation procedures except
1. Microinjection
2. Biolistics
3. Spooling
4. Electroporation
Select the incorrect statement.
1. Separation of DNA fragments occurs based on their size in agarose gel.
2. Blue-white selection involves insertional inactivation of β-galactosidase.
3. Treatment with ice-cold calcium can enhance efficiency of transformation in host cells.
4. Extension step during PCR is based on thermolabile nature of Taq polymerase.
DNA from Agrobacterium tumefaciens can be extracted through use of enzyme
1. Chitinase
2. Cellulase
3. Lysozyme
4. DNAse