| A. | Insertion of recombinant DNA into the host cell. | 
| B. | Cutting of DNA at specific location by restriction enzyme. | 
| C. | Isolation of desired DNA fragment. | 
| D. | Amplification of gene of interest using PCR. | 
| 1. | B, D, A, C | 2. | B, C, D, A | 
| 3. | C, A, B, D | 4. | C, B, D, A |