Manipulation of DNA in genetic engineering became possible due to the discovery of:

1. Restriction endonuclease

2. DNA ligase

3. Transcriptase

4. Primase

Subtopic:  Restriction Enzymes - Main Enzymes |
 91%
Level 1: 80%+
AIPMT - 2002
Hints

Restriction endonucleases:

1.  Are used in genetic engineering for ligating two DNA molecules.
2.  Are used for in vitro DNA synthesis.
3.  Are synthesized by bacteria as part of their defense mechanism.
4.  Are present in mammalian cells for the degradation of DNA when the cell dies. 

Subtopic:  Restriction Enzymes - Main Enzymes |
 56%
Level 3: 35%-60%
AIPMT - 2004
Hints

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain:

1. Lipase

2. protease

3. Exonuclease

4. Endonuclease

Subtopic:  Restriction Enzymes - Main Enzymes |
 78%
Level 2: 60%+
AIPMT - 2005
Hints

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