- 1(current)
Q. No.Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: Â General Design of an rDNA experiment |
 80%
Level 1: 80%+
Hints
Match List I with List II:
Choose the correct answer from the options given below:
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
| List I | List II |
| A. Chloramphenicol | I. Join the cut ends of DNA molecule |
| B. Ethidium bromide | II. Makes bacterial cell competent to take up DNA |
| C. DNA ligase | III. Selectable marker |
| D. Calcium | IV. Agarose gel staining |
1. A - (III); B - (IV); C- (II); D - (I)
2. A - (III); B - (IV); C- (I); D - (II)
3. A - (IV); B - (III); C- (II); D - (I)
4. A - (IV); B - (III); C- (I); D - (II)
Subtopic: Â General Design of an rDNA experiment |
 92%
Level 1: 80%+
Hints
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Â General Design of an rDNA experiment |
 59%
Level 3: 35%-60%
NEET - 2022
Hints
- 1(current)
Q. No.
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