A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:

1.  Human genes may have intron which bacteria cannot process
2.  Amino acid codons for humans and bacteria are different
3.  Human protein is formed but degraded by bacteria
4.  All of the above

Subtopic:  Tools | General Design of an rDNA experiment |
 61%

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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1.Charge only2.Size only
3.Charge-to-size ratio4.All of the above

Subtopic:  Selectable Markers: I | Selectable Markers: II | Host & Desired DNA | Tools |
 68%
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A bacterium adds methyl group to its DNA, by a process known as modification, in order to:

1.  Clone its DNA
2.  Turn its genes on
3.  Transcribe many genes simultaneously
4.  Protect DNA from its own restriction enzymes
Subtopic:  Tools: Enzymes: II | Tools |
 83%
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Which of the following cannot be used as a vector in rDNA technology?

1.Plasmid2.Phage DNA
3.Bacterium4.YAC

Subtopic:  Tools |
 59%
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:

1.  it allows a cell to recognize fragments produced by the enzyme
2.  the single-stranded ends serve as starting points for DNA replication
3.  the fragments will bond to other fragments with complementary single-stranded ends
4.  only single-stranded DNA segments can code for proteins
Subtopic:  Tools |
 78%

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Consider the following statements:

I:  Exonucleases remove nucleotides from the ends of DNA whereas, endonucleases make cuts at specific positions within DNA.
II:   To visualize pure DNA fragments, it is stained with ethidium bromide and seen in UV light.
III:  Plasmids and phage DNA are used as vectors in genetic engineering as they are extrachromosomal DNA molecules.

Which of the above statements are true?

1. I and II only 2. I and III only
3. II and III only  4. I, II, and III
Subtopic:  Tools: Enzymes: I | Tools |
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A DNA library is:

1.  information in a computer database about all the genes sequenced so far
2.  a collection of vectors in a molecular biology lab
3.  a collection of fragments of DNA that make up the entire genome of an organism
4.  all the restriction sites put together on a DNA fragment
Subtopic:  Introduction & History | Tools |
 67%

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Consider the following statements: 

I:  Ori is responsible for the copy number of the linked DNA.
II:  Transformation is a process through which a piece of DNA is introduced into a host bacterium. 
III:  Bacteriophages have very high copy numbers of their genome within the bacterial cell.

Which of the above statements are true?

1.  I and II only 2.  I and III only
3.  II and III only  4.  I, II, and III
Subtopic:  Tools: Vectors: I | Selectable Markers: I | Selectable Markers | Tools |
 79%
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