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Q. No.A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
| 1. | Human genes may have intron which bacteria cannot process |
| 2. | Amino acid codons for humans and bacteria are different |
| 3. | Human protein is formed but degraded by bacteria |
| 4. | All of the above |
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
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A bacterium adds methyl group to its DNA, by a process known as modification, in order to:
| 1. | Clone its DNA |
| 2. | Turn its genes on |
| 3. | Transcribe many genes simultaneously |
| 4. | Protect DNA from its own restriction enzymes |
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Which of the following cannot be used as a vector in rDNA technology?
| 1. | Plasmid | 2. | Phage DNA |
| 3. | Bacterium | 4. | YAC |
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It is useful to use restriction enzymes in rDNA experiments that produce sticky ends in the resultant fragments because:
| 1. | it allows a cell to recognize fragments produced by the enzyme |
| 2. | the single-stranded ends serve as starting points for DNA replication |
| 3. | the fragments will bond to other fragments with complementary single-stranded ends |
| 4. | only single-stranded DNA segments can code for proteins |
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Consider the following statements:
| I: | Exonucleases remove nucleotides from the ends of DNA whereas, endonucleases make cuts at specific positions within DNA. |
| II: | To visualize pure DNA fragments, it is stained with ethidium bromide and seen in UV light. |
| III: | Plasmids and phage DNA are used as vectors in genetic engineering as they are extrachromosomal DNA molecules. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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A DNA library is:
| 1. | information in a computer database about all the genes sequenced so far |
| 2. | a collection of vectors in a molecular biology lab |
| 3. | a collection of fragments of DNA that make up the entire genome of an organism |
| 4. | all the restriction sites put together on a DNA fragment |
Consider the following statements:
| I: | Ori is responsible for the copy number of the linked DNA. |
| II: | Transformation is a process through which a piece of DNA is introduced into a host bacterium. |
| III: | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
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