| Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. |
| Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. |
| List-I | List-II | ||
| A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm |
| B. | Cry IAb | II. | ADA deficiency |
| C. | Cry IAc | III. | Emphysema |
| D. | Enzyme replacement therapy | IV. | Corn borer |
| 1. | Bt toxin requires acidic pH for activation |
| 2. | Bacillus has protective mechanism against it |
| 3. | In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form |
| 4. | The protoxin requires protein conjugate for its activation |
| A. | The inactive toxin converted into active form due to alkaline pH of gut of insect |
| B. | Bacillus thuringiensis produce crystals with toxic insecticidal proteins. |
| C. | The alkaline pH solubilises the crystals. |
| D. | The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. |
| E. | The toxin proteins exist as inactive protoxins in bacteria. |
| List-I | List-II | ||
| A. | Genetically engineered Human Insulin | I. | Gene therapy |
| B. | GM Cotton | II. | E.Coli |
| C. | ADA Deficiency | III. | Antigen-antibody interaction |
| D. | ELISA | IV. | Bacillus thuringiensis |
| 1. | creating pores in the midgut |
| 2. | damaging the respiratory system |
| 3. | degenerating the nervous system |
| 4. | altering the pH of body fluids |
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (BT) is resistant to:
| 1. | Fungal diseases | 2. | Plant nematodes |
| 3. | Insect predators | 4. | Insect pests |
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
| (a) | (b) | (c) | (d) | |
| 1. | (iv) | (iii) | (i) | (ii) |
| 2. | (iii) | (ii) | (iv) | (i) |
| 3. | (iii) | (iv) | (i) | (ii) |
| 4. | (ii) | (iv) | (iii) | (i) |
Match the following columns and select the correct option.
| Column-I | Column-II | ||
| a. | Bt cotton | (i) | Gene therapy |
| b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
| c. | RNAi | (iii) | Detection of HIV infection |
| d. | PCR | (iv) | Bacillus thuringiensis |
| a | b | c | d | |
| 1. | (iii) | (ii) | (i) | (iv) |
| 2. | (ii) | (iii) | (iv) | (i) |
| 3. | (i) | (ii) | (iii) | (iv) |
| 4. | (iv) | (i) | (ii) | (iii) |