A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:

1. It includes the centromere
2. It becomes a part of chromosome
3. It does not contain any intron sequences
4. It is a single stranded polynucleotide

Subtopic:  Cloning Vector |
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:

1. Telomeric sequence 2. Multiple cloning sites
3. Ori 4. Selectable marker

Subtopic:  Cloning Vector |
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In recombinant DNA experiments, a vector:

1. carries DNA into a new cell 
2. links together newly joined fragments of DN
3. makes millions of copies of a specific segment of DNA
4. separates fragments of DNA by their length and electrical charges
Subtopic:  Cloning Vector |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?

1. Ori 2. rop
3. Selectable markers 4. Promoter in the insert
Subtopic:  Cloning Vector | Obtaining Copy of Gene from Donor DNA |
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What is true for the plasmids used as cloning vectors in recombinant procedures?

1. Ori is responsible for controlling the copy number of linked DNA
2. The bacteria lacking R plasmids are resistant to antibiotics
3. The vector must have many sites for the same restriction enzyme
4. They can carry largest inserts amongst the available cloning vectors
Subtopic:  Cloning Vector |
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The most important feature in a plasmid to serve as a vector in gene cloning experiment is:

1. Origin of replication (ori)

2. Presence of a selectable marker

3. Presence of sites for restriction endonuclease

4. Its size

Subtopic:  Cloning Vector |
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Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies

Subtopic:  Cloning Vector |
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In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:

1. 1 and 2 2. 3 and 4
3. 6 and 7 4. 5 and 8
Subtopic:  Cloning Vector |
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A cloning vector has two antibiotic resistance genes: tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinant transformants would survive on the medium containing:

1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin
Subtopic:  Cloning Vector |
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The Ti plasmid, used in transformation of plant cells, is found in:

1. Agrobacterium tumefaciens 2. Escherichia coli
3. Bacillus thuringiensis 4. Xanthomonas citri
Subtopic:  Cloning Vector |
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