Match column I with column II and choose the correct option:
Column I Column II
a. Salmonella typhimurium (i) Source of EcoRI
b. Bacillus thuringiensis (ii) Used in developing transgenic tobacco plant
c. Agrobacterium
tumefaciens
(iii) Used in creating first
recombinant DNA
d. Escherichia coli (iv) Gene used in creating biopesticide

1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic:  Restriction Enzymes - Main Enzymes | Transforming Plant & Animal Cell | Restriction Enzymes: Historical Background |
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Complete the analogy and select the correct option.
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
Subtopic:  Restriction Enzymes - Main Enzymes |
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If restriction endonuclease cuts the DNA a little away from the centre of the palindromic site, then what would happen? 
(a) The DNA fragments obtained will have 'sticky ends'
(b)Construction of rDNA molecule would not be possible 
(c) Resultant DNA fragments with overhanging streches can be ligated with the help of DNA ligase 
Choose the correct option 
1. (a) and (b) are correct 
2. (a) and (c) are correct 
3. (b) and (c) are correct 
4. (a), (b) and (c) are correct 
Subtopic:  Restriction Enzymes - Main Enzymes |
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Assertion(A): Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason(R): Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic:  Restriction Enzymes - Main Enzymes | Cloning Vector | Introduction & History |
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Assertion(A): The restriction endonucleases can recognize palindromic sequences.
Reason(R): These sequences read the same in both directions of the two complementary DNA strands.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic:  Restriction Enzymes - Main Enzymes |
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Given below are two statements: One is labeled as Assertion (A) and the other is labeled as Reason (R) 

Assertion (A):  Restriction enzyme is a type of endonuclease 
Reason (R):  Restriction enzyme cuts the two stands of DNA at specific positions within the DNA 


In the light of the above statements, choose the correct answer from the options given below: 

1. (A) is correct but (R) is not correct.
2. (A) is not correct but (R) is correct.
3. Both (A) and (R) are correct and (R) is the correct explanation of (A).
4. Both (A) and (R) are correct but (R) is not the correct explanation of (A).
Subtopic:  Restriction Enzymes - Main Enzymes |
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