Use of primers, occurs in which step of PCR?

1.     Annealing

2.     Extension

3.     Denaturation

4.     Both extension and Annealing

The ultimate aim of maximum recombinant DNA technologies is

1.   To multiply DNA

2.   To express DNA

3.   To multiply mRNA

4.   To express protein

Which of the following processes is not associated with the protein produced by recombinant DNA?

1.      Restriction Digestion

2.      Expression

3.      Optimization

4.     Purification

What does continuous culture mean?

1. Where DNA and protein getting expressed continuously

2. Where used medium is drained out and the fresh medium is added from another side

3. Where cells are producing protein for one week continuously

4. Where the production of recombinant DNA is continuing without interference.

Continuous culture has

1.      Cells in their physically active phase

2.      Lag phase

3.      Both A and B

4.     Exponential phase

Continuous culture leads to

1. Smaller biomass

2. Higher yields of desired proteins

3. Both 1 and 2

4. Higher yields of recombinant DNA

The volume of bioreactors cannot be

1.      20 L

2.      100L

3.      150L

4.     200L

Bioreactors don't provide which of the conditions?

1. Salts

2. Vitamins

3. Cells

4. Oxygen

The most commonly used bioreactors are

1. Stirring type

2. Sparged type

3. Aerobic type

4. Both 2 and 3

The cylindrical body or curved base of a bioreactor actually facilitates

1. The handling and maintenance of bioreactor

2. mixing

3. better oxygen transport

4. more accumulation of product