Which of the following is a tool of RDT?

1.      PCR

2.      Gel Electrophoresis

3.      Cloning

4.     Restriction enzyme

In the year 1963, the two enzymes responsible for inhibiting the growth of bacteriophage in E. coli were isolated. One of them was

1.      Ligase

2.      Polymerase

3.      Molecular scissor

4.     Antibiotic resistance enzyme

Hind II

1.      First restriction endonuclease

2.      Was characterized 7 years later

3.      Specific sequence of 5 base pairs

4.     All of these

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1.      700; 350

2.      900; 230

3.      600; 270

4.     550; 210

Which of the following is correct representation of the name of enzyme?

1.      ECORI

2.      EcoRI

3.      ECoRI

4.     ECoR1

EcoRI comes from

1.      Escherichia coli RY13

2.      Escherichia coli RY16

3.      Escherichia coli RY19

4.     Escherichia coli RY12

In EcoRI, here I stands for

1.      Order in which strains are discovered

2.      Order in which enzymes are isolated

3.      Order in which enzymes are isolated from that strain

4.      Order in which strains are reported in microbiology

Restriction endonuclease

1.      Directly recognizes a specific sequence

2.      Makes the cut at the sugar-phosphate core.

3.      Comes under nuclease

4.      Both 1 and 3

Restriction endonuclease cuts

1. At palindromic sequences for some enzymes not for all

2. After scanning and recognition of specific sequence in a DNA strand

3. Cuts the strand exactly at the centre

4. All of these

Restriction enzyme EcoRI cuts

1.      Between bases G and A

2.      Cuts between GAATTC

3.      Cuts between G and A  at GAATTC

4.      Both 1 and 3