Insertional inactivation of the lac Z gene forms-

1. Blue recombinant colonies
2. Colourless recombinant colonies
3. Fluorescent green colonies
4. There is no relation between the lac Z gene and colour of the colony.

Subtopic:  Selectable Markers: III | Selectable Markers |
 71%
From NCERT
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A reporter enzyme used in biotechnology is-

1. Green Fluorescent Protein
2. Luciferase
3. b Galactosidase
4. All of the above
Subtopic:  Selectable Markers: III | Selectable Markers |
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Downstream processing does not include

(1) Quality control

(2) Clinical trials

(3) Product purification

(4) Sampling from bioreactor

Subtopic:  Large Scale Production: II | Large Scale Production |
 64%
From NCERT
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Which is not a component of the vector pBR322?

1. Chloramphenicol selectable marker
2. Replication proteins
3. Origin of replication
4. Cloning site for BamHI enzyme
Subtopic:  Tools: Vectors: II | Tools |
 75%
From NCERT
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Which among the following is a cloning vector?

1. EcoRI
2. BamHI
3. pUC18
4.Sal I

Subtopic:  Tools: Vectors: II | Tools |
From NCERT
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Two bacteria found to be very useful in genetic engineering are

(1) Diplococcus and Streptomyces

(2) Rhizobium and Xanthomonas

(3) Vibrio and Escherischia

(4) Escherischia and Agrobacterium

Subtopic:  Tools: Vectors: II | Host & Desired DNA: I | Host & Desired DNA | Tools |
 97%
From NCERT
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Which of the following would you choose as the safest and least cumbersome selectable marker-

1. Ampicillin resistance gene
2. Tetracyclin resistance gene
3. Kanamycin resistance gene
4. b Galactosidase gene
Subtopic:  Selectable Markers: III | Selectable Markers |
 69%
From NCERT
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Recombinant DNA is formed in nature during-

1. Crossing over at pachytene
2. Integration of plasmid to form episome
3. Integration of phage DNA to form prophage
4. More than one is correct.
Subtopic:  Tools: Vectors: I | Tools |
 69%
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Southern Blotting essentially makes use of a

1. Buffer 2. Electrodes
3. Agarose gel 4. All of the above
Subtopic:  Process of Biotech |
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The native charge on DNA molecules is

1. Positive 2. Negative
3. Neutral 4. DNA is an ampholyte
Subtopic:  Process of Biotech |
 95%
From NCERT
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