The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

The DNA molecule to which the gene of interest is integrated for cloning is called:

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

(1) Non-recombinant bacteria containing $\beta$-galactosidase

(2) Insertional inactivation of $\alpha$-galactosidase in non-recombinant bacteria

(3) Insertional inactivation of β-galactosidase in recombinant bacteria

(4) Inactivation of glycosidase enzyme in recombinant bacteria

The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme

2. rop-reduced osmotic pressure

3. Hind III, Eco RI -selectable markers

4. amp^R, tet^R-antibiotic resistance genes

Which one of the following is a case of wrong matching ?

1. Somatic hybridisation - Fusion of two diverse cells

2. Vector DNA - Site for tRNA synthesis

3. Micropropogation - In vitro production of plants in large numbers

4. Callus - Unorganised mass of cells produced in tissue culture

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature