A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
| 1. | human genes may have intron which bacteria cannot process |
| 2. | amino acid codons for humans and bacteria are different |
| 3. | human protein is formed but degraded by bacteria |
| 4. | All of the above |
Inducing a cloned eukaryotic gene to function in a prokaryotic host can be difficult sometime. The presence of long non-coding introns in eukaryotic genes may prevent correct expression of these genes in prokaryotes, which lack RNA-splicing machinery.
