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Choose the mismatch,
(1) Phagemid - Bacteriophage and plasmid
(2) Transposons - Mobile genetic elements
(3) Shuttle vector - yEP
(4) Ti plasmid -Agrobacterium rl›izogenes

Which fluorescent dye is used for staining nucleic acid during gel electrophoresis?
(1) EDTA
(2) Ethidium bromide
(3) Ninhydrin
(4) Eosine

Which rDNA product is used in the treatment of internal clot?
(1) Stomatostain
(2) Relaxin
(3) Streptokinase
(4) Growth hormone

A. Separation and purification after the biosynthesis of product is included in downstream processing.
R. Downstream processing is a highly labour intensive step requiring high level of quality control.
(1) If both Assertion & Reason are true and the reason is the correct explanation of the assertion
(2) If both Assertion & Reason are true but the reason is not the correct explanation of the assertion
(3) If Assertion is true statement but Reason is false.
(4) If both Assertion and Reason are false statements

Find the correct match with respect to genetically modified plants / organism.
     Column I                                  Column II
a. Flavr Savr                      (i) First transgenic cow
b. Golden rice                    (ii) Cry genes
c. Bt cotton                         (iii) Pro-vitamin "A"
d. Roise                              (iv) Inhibition of polygalacturonase
(1) a(iv), b(iii), c(ii), d(i)
(2) a(iii), b(iv),c(ii),d(i)
(3) a(iv), b(ii), c(iii), d(i)
(4) a(ii), b(iii),c(iv),d(i)

What happens if we ligate a foreign DNA at the Bam $H_1$ site of Lax Z' $\beta$-galactosidase gene of pUC-19 cloning vector?
(1) There is insertional inactivation of Lac Z' gene
(2)  $\beta$-galactosidase enzyme is not produced
(3) Recombinants appear as white coloured colonies on X-gal containing medium
(4) All of these

The most commonly used restriction endonuclease enzymes in biotechnology are _____________.
The first restriction endonuclease enzyme discovered was ______________.
Choose the correct option to fill up the blanks
(1) EcoR I; Hindi I
(2) Type I; EcoR I
(3) Type II; EcoR I
(4) Type II; Hindi II

A. Selection of recombinants due to insertional inactivation of antibiotic resistant gene is a cumbersome process.
R. It requires simultaneous plating on two plates having two different antibiotics.
(1) bolh Assertion & Reason are true and the reason Is the correct explanation of the assertion
(2) Both Assertion & Reason are true but the reason is not the correct explanation of the assertion
(3) Assertion is true statement but Reason is false
(4) both Assertion and Reason are false statements

The vector commonly used for sequencing human genome
(1) YAC
(2) Plasmid
(3) MV Vector
(4) M13 vector

What is common between Eco RI and Hind II?
(1) Obtained from same source
(2) Produce sticky ends
(3) Hyrolyse phosphodiester bonds
(4) Produce flush ends

The mechanism of intake of DNA fragments from the surrounding medium by a cell is called
(1) Transformation
(2) Transduction
(3) Both (1) and (2)
(4) Conjugation

The PCR technique was developed by
(1) Kary Mullis
(2) Kohler
(3) Milstein
(4) Altman

The process of binding primer to the denatured strand is called
(1) Denaturation
(2) Annealing
(3) Melting
(4) Electroporation

A plasmid vector should have which of the following properties?
(i) MCs
(ii) Multiple Ori
(iii) Small Size
(iv) Selectable markers
(1) (i), (ii) & (iii)
(2) (ii), (iii) & (iv)
(3) (i), (ii) & (iv)
(4) (i), (iii) & (iv)

Taq polymerase is used in PCR because of its
(1) Low thermal stability
(2) High fidelity
(3) High speed
(4) High thermal stability

Which of the restriction enzymes are commonly used in rDNA technology?
(1) Type I
(2) Type II
(3) Type III
(4) Type IV

In a PCR, which of the following components is not required?
(1) Nucleotide precursors
(2) A primer containing 3' - OH
(3) DNA helicase to separate the strands
(4) DNA polymerase to catalyse the reaction

A plasmid has two antibiotic - resistance genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Host cells that have taken up the recombinant DNA are the one that
(1) Are blue and can grow on plates with antibiotics
(2) Can grow on plates with ampicillin but not with tetracyline
(3) Can grow on plates with tetracycline but not with ampicillin
(4) Cannot grow with any antibiotics

A thermostable DNA polymerase is isolated from
(1) E. coil
(2) Salmonella
(3) Thermus coccus
(4) Thermus aquaticus

Complete the analogy:
Molecular scissor : Restriction endonuclease : : Molecular glue : X
(1) Alkaline phosphatase
(2) Acid phosphatase
(3) Taq polymerase
(4) DNA ligase

Bacterial resistance to antibiotics is a generic trait carried in the bacterial
(1) Intron
(2) Chromosome
(3) Plasmid
(4) Centromere

The vector used to produce Humulin was
(1) Cosmid
(2) Plasmid pBR322
(3) Ti plasmid
(4) Escherichia coli

To prevent self-ligation of vector DNA and to increase the frequency of production of recombinant DNA technology which enzyme is used?
(1) Reverse transcriptase
(2) Alkaline phosphatases
(3) Restriction endonuclease
(4) Acid phosphatases

Animal biotechnoloy involves
(1) Production of valuable products in animals using rDNA technology
(2) Rapid multiplication of animals of desired genotypes
(3) Alteration of genes to make the animal more desirable
(4) All of these

Recombinant proteins are
(1) Proteins synthesized in animals
(2) Proteins synthesized by transgene in heterologous host cell by rDNA technology
(3) Proteins synthesized in cells that are produced by protoplast fusion
(4) Proteins synthesized in mutated cell lines

A bacterial cell is transformed by using any of the following methods, except
(1) Heat shock method
(2) Electroporation
(3) Biolistics
(4) Via bacteriophage

The role of ethidium bromide in gel electrophoresis is that
(1) It induces a negative charge on all DNA strands
(2) It makes DNA strands visible as orange coloured strips
(3) It ensures separation of DNA strands on the basis of charge and mass
(4) It removes the gel associated with DNA strands

Mark the statement that is incorrect for the enzyme(s) involved in biotechnology
(1) Lysozymes are used to dissolve the bacterial cell walls
(2) DNA ligase completes the DNA backbone by forming covalent bonds
(3) Restriction enzymes and DNA ligase can be used together to join lengths of DNA from different sources
(4) DNA polymerases help in synthesis of complementary DNA and reverse transcriptase helps in in-vitro synthesis of DNA

A good vector is one
(1) That makes transformation process complex
(2) That maintains its individuality in host cell
(3) That is easy to isolated and to purify
(4) Generates few copies of host DNA

A. DNA fragments can be separated on the basis of their sizes.
R. Gel electrophoresis technique does not depend on the charge on DNA fragments.
(1) Both Assertion & Reason are true and the reason is the correct explanation of the assertion.
(2) Both Assertion & Reason are true but the reason is not the correct explanation of the assertion.
(3) Assertion is true statement be Reason is false.
(4) Both Assertion and Reason are false statements.

DNA fragment can be joined together (end to end) using DNA ligases when they have been
(1) Cut by the same restriction enzyme
(2) Cut by the different restriction enzyme
(3) Treated by alkaline phosphatase
(4) Combined with plasmid components

Small oligonucleotides capable of recognizing complementary sequence are known as
(1) cDNA
(2) Hybridoma
(3) Repetitive DNA
(4) Molecular probe

When a typical restriction enzyme cuts a DNA molecule, it leaves single stranded portions get the ends. These overhanging stretches are called sticky ends on each strand. They are so named because
(1) They serve as the starting points for DNA replication
(2) They enable the researchers to use the fragments as molecular probes
(3) They form hydrogen bonds with their complementary cut counter parts
(4) The ends are called as chemical scapels

The construction of the first artificial recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
(1) Escherichia coli
(2) Agrobacterium tumefaciens
(3) Salmonella typhimurium
(4) Bacillus amyloliquefaciens

If the bacterial colonies do not produce any colour. In the presence of chromogenic substance it confirms
(a) Insertional inactivation of $\beta$ galactosidase enzyme
(b) Recombinant
(c) Activation of $\beta$ galactosidase enzyme
(d) Non-recombinants
Mark the correct choice
(1) (a) & (b)
(2) (c) & (d)
(3) (a) & (c)
(4) (a) & (d)

All the following are examples of vectorless gene transfer, except
(1) Particle gun
(2) Microinjection
(3) Cosmid
(4) PEG

Genetically modified plants have been useful in many ways as genetic modification is responsible for following except
(1) Made crops more tolerant to abiotic stresses
(2) Reduced reliance on chemical pesticides
(3) Enhanced nutritional value of food
(4) Decreased efficiency of mineral usage by plants

Yeast have been extensively used for the functional expression of genes because these offer several advantages except
(1) They are simple eukaryotic organism
(2) Genetically well characterized
(3) Grown readily in both small culture vessel and large scale bio-reactors
(4) Chitinous cell wall

Molecular scissors cut specific pallindromic sequences except
(1) DNA sequences
(2) Methylated DNA sequences
(3) Between phosphate and sugar in DNA only
(4) Both (1) & (2)

Restriction endonucleases are the most widely used in recombinant DNA technology. They are obtained from
(a) Bacteriophage
(b) Bacterial cells
(c) Plasmids
(d) All prokaryotic cells
(1) (a) only
(2) (a) and (b)
(3) (b) only
(4) (b) and (d)

Refer to this sketch of a gel with four samples of DNA. Which well contained DNA that did not undergo restriction digestion and which lane contains the smallest piece of DNA?

(1) 3 & 2
(2) 2 & 3
(3) 3 & 4
(4) 2 & 1

A virus with ssDNA is discovered.  Its DNA is amplified by running 10 cycles of PCR. How many dsDNA strands will be formed at the end?
(1) 10
(2) 512
(3) 1024
(4) 1023

Which of the following are critical research areas of biotechnology?
(1) Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme
(2) Creating optimal conditions through engineering for a catalyst to act
(3) Downstream processing technologies
(4) All of these

If the recombinants grow in ampicillin containing medium and not on that containing tetracycline. It means that foreign DNA is inserted in
(1) Site of origin of replication
(2) Rop site
(3) Tetracycline selective marker site
(4) Ampicillin selective marker site

In order to force bacteria to take up the plasmid, the bacterial cells must first be made competent to take up DNA. This is done by treating them with specific.
(1) Concentration of divalent cation such as calcium
(2) Incubating cell on ice, heat shock and back on ice
(3) Low concentration of monovalent ions followed by increase in temperature
(4) Both (1) & (2)

As the DNA is enclosed within the membrane so the membrane has to be broken to release DNA along with other macromolecules. It can be achieved by treating plant cell by
(1) Lysozyme
(2) Lipases
(3) Chitinase
(4) Cellulase

What is the sequence of steps in PCR?
(1) Amplification, insertion, polymerisation
(2) Denaturation, annealing, chain elongation
(3) Annealing, chain termination, denaturation
(4) Annealing, amplification, polymerisations

Which of following method is unrelated to downstream processing steps?
(1) Centrifugation
(2) Fermentation
(3) Chromatography
(4) Distillation

Which restriction enzymes are used for selectable marker $te{t}^R$ on pBR 322?
(1) Pvu I, Pst I
(2) Pvu I, BamHI
(3) Sal I, BamHI
(4) Pst I, Sal I

The 'sticky ends' of the DNA can be paired a joined by
(1) Methylase
(2) Ligase
(3) X-gal
(4) Alkaline phosphatase

Genetic engineering has been used to do all of the following except
(1) Make plants more resistant to frost
(2) Make plants more resistant to diseases
(3) Improve the nutritional balance of plants
(4) All of these are correct

Transgenic bacteria have been developed and are used for achieving all the following except
(1) Production of insulin
(2) Production of human growth hormone
(3) Clearing oil slicks in oceans
(4) To test the safety of polio vaccine

Restriction endonuclease generated DNA fragments separated by gel electrophoresis and blot transferred onto a membrane filter are probed with radioactive DNA fragment. This procedure is called
(1) Gene cloning
(2) The southern technique
(3) Gene mapping
(4) PCR

Which of the following restriction site is present in rop gene of pBR 322?
(1) BamHI
(2) Pvu I
(3) Pvu II
(4) Sal I